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The presence of a clinical prostatic carcinoma is an absolute contraindication for HRT and should be carefully excluded by PSA, rectal examination and, eventually, biopsy before starting any therapy.45 There is also no clear recommendation for men successfully treated for prostate cancer who would be potential candidates for testosterone substitution after a ‘prudent’ interval if there is no clinical or laboratory evidence of residual cancer.45 Therefore, the increased intratesticular levels of apelin found in this study may have partly contributed to sunflower oil- and omega-3-induced decreases in male reproductive hormones and increases in antioxidant enzyme levels. This study was undertaken to elucidate the effect of omega-3 and omega-6 supplementation on the levels of different adipokines and cytokines, as well as the antioxidant system, in relation to male reproductive hormones and testicular functions. 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