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It remains a major health threat, killing over 1 million people every year worldwide. Available confirmatory investigations include HSV DNA detection by polymerase chain reaction (PCR), rapid direct immunofluorescence assays (DFAs), serologic tests showing immunoglobulin G (IgG)- and immunoglobulin M (IgM)-specific antibody responses, and presence of multinucleated giant cells on a Tzanck smear (these are also seen with varicella-zoster virus infection)”.