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Negative controls depicted in Figures S4C and S5C demonstrate that we did not detect DsRed, anti-human mitochondria or anti-human mtTFA signal in samples from mice that were treated with cisplatin but did not receive the human mitochondria. 3D orthogonal slice views confirm that the human mitochondria are internalized by cells in the pia mater (Figure 5B') and glial limitans (Figure 5B''). Additionally, the administered mitochondria were seen entering the brain from the pia mater - closely surrounding the brain parenchyma, where they were present at 30 min and even 18 h after administration, interacting with GFAP+ cells (Figure 5A-C). The orthogonal slice views demonstrate that the human mitochondria are located well within the cells (Figure 4C). At 30 min after nasal administration, a substantial number of DsRed+ anti-human mitochondria+ mitochondria were located at the ventricles (Figure 4B). 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